DiaGreen® Media Calculator

Culture Target (Diatoms vs Green Algae): Volume Unit | Total Volume:

Base f/2 Chemical Inventory Overview

Macronutrients:

NaNO₃ (Sodium Nitrate)
NaH₂PO₄ (Sodium Phosphate Monobasic)

Silicate (Diatoms Only):

Na₂SiO₃ (Sodium Metasilicate)

Trace Metals:

FeCl₃ (Ferric Chloride) & Na₂EDTA (Disodium EDTA)
CuSO₄ (Cupric Sulfate), ZnSO₄ (Zinc Sulfate), CoCl₂ (Cobalt Chloride)
MnCl₂ (Manganese Chloride), Na₂MoO₄ (Sodium Molybdate)

Vitamins:

Thiamine · HCl (B1), Biotin (H), Cyanocobalamin (B12)

⚠️ Advanced Tuning: Hydration & Strength Adjustments

Adjust specific hydration states based on your chemical inventory. The engine will auto-correct molecular weights.

Overall Concentration Multiplier:

Sodium Phosphate (NaH₂PO₄):

Sodium Metasilicate (Na₂SiO₃):

Ferric Chloride (FeCl₃):

Disodium EDTA (Na₂EDTA):

Cupric Sulfate (CuSO₄):

Zinc Sulfate (ZnSO₄):

Manganese Chloride (MnCl₂):

Cobalt Chloride (CoCl₂):

Sodium Molybdate (Na₂MoO₄):

⬅ Back to Quick References

*Base formulation adapted from Guillard, R.R.L. and Ryther, J.H. (1963). Studies of marine planktonic diatoms. Canadian Journal of Microbiology, 9(2), pp.229-239.

Disclaimer & DiaGreen® Culture Integrity

Eiyarkai Three Life Sciences LLP is the proud manufacturer and supplier of DiaGreen® algae starter cultures, meticulously maintained to be strictly ciliate-free and vibrio-free.

While the media formulation shipped alongside DiaGreen® cultures features a proprietary modification of the f/2 medium specifically optimized to preserve cellular vitality and mitigate stress during transportation, the standard published Guillard’s f/2 media (calculated above) is entirely sufficient and highly recommended for general, large-scale facility cultivation.

This calculator is provided as a supplementary decision-support tool. Formulation adjustments, scaling, and sterilization protocols remain at the professional discretion of the facility technician. Eiyarkai Three Life Sciences LLP assumes no liability for chemical miscalculations or facility culture crashes.